Nicotine (Cotinine) Saliva Self-Test

Nicotine (Cotinine) Saliva Self-Test – result in 10 minutes

If you want a clear, practical indication of recent nicotine exposure (from smoking, vaping, or other nicotine products), this saliva self-test can help. It detects cotinine—the primary metabolite of nicotine—in saliva and provides a straightforward screening result within minutes, without the need for a lab visit.

What the test measures

This test screens for cotinine in saliva. Because cotinine remains measurable longer than nicotine itself, it is commonly used as an indicator of recent nicotine intake. The outcome is a screening indication (above/below the cut-off), not a precise concentration.

Why this test is useful

• Gives a fast screening result you can use for personal monitoring
• Uses a simple saliva swab—no blood sampling
• Requires no additional equipment
• Provides a clear readout with a control line to confirm the test ran correctly
• Reported reliability: 98% (as stated in the product specifications)

How to read the result (important)

This is a competitive lateral-flow test, so interpretation differs from many other self-tests:

• The control line (C) must appear. If it does not, the test is invalid.
• Test line (T) visible = negative (below the cut-off).
• No T line = positive (above the cut-off).

Follow the included instructions carefully for correct interpretation.

How to use

For the most meaningful result:

1. Avoid eating, drinking, chewing gum, vaping, or smoking for 10–15 minutes before testing.
2. Swab along the gum line 10–15 times, then place the swab under your tongue until saliva appears in the test window (about 60 seconds).
3. Add the buffer as instructed.
4. Results typically appear within 3–10 minutes. For consistency, read the result at 10 minutes and do not interpret after 20 minutes.

Who this test is for

This test is suitable for anyone who wants a quick at-home screening for recent nicotine use—for example, people tracking smoking/vaping habits or monitoring progress while reducing or quitting.

What’s included

• 1 test cassette
• 1 saliva swab/collector
• 1 buffer tube
• Instruction manual (Dutch/English availability depends on the batch)

Important information

• Single-use, in-vitro diagnostic test
• Store at 2–30°C (do not freeze)
• A screening result is not a medical diagnosis
• If the outcome is important or the result is unclear, repeat the test and/or confirm via a laboratory method